.alpha.-1, 6-glucosidase and process for producing the same

Details .alpha.-1, 6-glucosidase and process for producing the same .alpha.-1, 6-glucosidase and process for producing the same

C - Chemistry, Metallurgy

12

N

195/123, 195/33.

C12N 9/44 (2006.01)

Patent

CA 1285896

ABSTRACT OF THE DISCLOSURE A novel .alpha.-1,6-glucosidase having the following properties: (1) Action: The enzyme acts on an .alpha.-1,6- glucoside bond to produce linear amylose; (2) Substrate specificity: The enzyme has the following relative activity to polysac- charides: Image (3) Km value and Vmax to pullulan: The enzyme has Km value of 0.14 mg/ml and Vmax of 70.0 µmol/min/mg protein; (4) Optimum pH: 5.0 to 5.5; (5) pH stability: The enzyme is stable in the pH range of 4.5 to 6.5 for 30 minutes at 40°C and in the pH range of 5.0 to 6.0 for 30 minutes at 50°C; (6) Optimum temperature: Approximately 55°C; (7) Thermal stability: Treatment at pH 4.5 for 30 minutes at different temperatures indicates that the enzyme retained 80% of the initial activity at 40°C and 10% thereof at 60°C; (8) Influence of inhibitor: The enzyme is inhibited by at least 70% by p-chloro- mercuribenzoic acid or sodium dodecylsulfate, but is not inhibited by o-phenanthroline or potassium ferricyanide; (9) Influence of metal salts: The enzyme is negligibly affected by Ni2+, Ba2+, Zn2+, Cd2+ or Mn2+; is inhibited by Fe3+; and loses activity in the presence of Hg2+ or Ag2+ ; and (10) Molecular weight; About 95,500 (measured by SDS electrophoresis).

547913

Affiliated with

Also associated with

Rating

.alpha.-1, 6-glucosidase and process for producing the same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with .alpha.-1, 6-glucosidase and process for producing the same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and .alpha.-1, 6-glucosidase and process for producing the same will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1229619