Amidase activity polypeptides; genetic tools and host...

Details Amidase activity polypeptides; genetic tools and host... Amidase activity polypeptides; genetic tools and host...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/80 (2006.01) C12P 7/44 (2006.01)

Patent

CA 2153793

Novel polypeptides having amidase activity for hydrolyzing amides into carboxylates, and having a greater enzymatic activity for ammonium adipamate than for adipamide. A DNA sequence coding for a polypeptide having amidase activity for hydrolyzing amides into carboxylates, an analogue thereof resulting from genetic code degeneration, or a DNA sequence hybridizing with one of said sequences or a fragment thereof and coding for a polypeptide having amidase activity, are disclosed. Germs suitable for producing said polypeptides are also disclosed.

La présente invention concerne de nouveaux polypeptides à activité amidase, capables d'hydrolyser les amides en carboxylates, caractérisés en ce qu'ils possèdent une activité vis-à-vis de l'adipamate d'ammonium supérieure à celle qu'ils ont vis-à-vis de ladipamide. Elle concerne, également,une séquence d'ADN codant pour un polypeptide ayant une activité amidase, capable d'hydrolyser les amides en carboxylates, un analogue de cette séquence résultant de la dégénérescence du code génétique, ou une séquence d'ADN hybridant avec l'une de ces séquences ou un fragment de celles-ci et codant pour un polypeptide ayant une activité amidase. Enfin, l'invention couvre les germes aptes à produire les susdits polypeptides.

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