C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/48 (2006.01) C07K 14/61 (2006.01) C12N 15/18 (2006.01) C12N 15/57 (2006.01)
Patent
CA 2282734
The present invention relates to a process for removing methionine (Met) residue at N-terminus of proteins specifically. Particularly, the present invention relates to an aminopeptidase which is purified from Bacillus licheniformis removes a methionine residue from N-terminus of peptide and proteins. And the present invention relates to a process for preparing a natural type protein from proteins produced in microorganisms by recombinant DNA technology. Various kinds of natural type proteins such as human growth hormone (HGH) can be prepared massively and easily by the process of the present invention.
L'invention concerne un procédé de suppression du reste méthionine (Met) au niveau du N-terminal de protéines. Elle se rapporte, notamment à une aminopeptidase qui est purifiée à partir de Bacillus licheniformis, qui supprime un reste méthionine du N-terminal de peptides et de protéines. Elle porte encore sur un procédé de préparation d'une protéine de type naturel à partir de protéines produites dans des micro-organismes par une technologie de recombinaison de l'ADN. Divers types de protéines de type naturel, telle que l'hormone de croissance humaine, (HGH) peuvent se préparer massivement et facilement au moyen du procédé de l'invention.
Han Kyuboem
Kim Se Hoon
Lee Seung Joo
Lee Young Phil
Park Soon Jae
Lg Chemical Ltd.
R. William Wray & Associates
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