An enzyme-based system and sensor for measuring acetone

Details An enzyme-based system and sensor for measuring acetone An enzyme-based system and sensor for measuring acetone

C - Chemistry, Metallurgy

12

N

C12N 9/04 (2006.01) A61B 5/08 (2006.01) A61B 10/00 (2006.01) C12M 1/40 (2006.01) C12N 11/00 (2006.01) C12P 7/04 (2006.01) C12P 7/28 (2006.01) C12Q 1/26 (2006.01) G01N 33/15 (2006.01) G01N 33/64 (2006.01) A61B 5/00 (2006.01)

Patent

CA 2465048

Described are enzyme systems specific for acetone and methods of using these enzyme systems to detect acetone in biological or environmental samples. Biosensors containing these enzyme systems are disclosed, in which detection of acetone may be achieved by linking electrochemical, photometric, or other detection means to one or more acetone-specific enzyme reactions or pathways. Methods of using such acetone-specific biosensors include subject management of weight loss, disease detection, and bioavailability monitoring of therapeutics.

L'invention se rapporte à des systèmes d'enzymes propres à l'acétone et à des procédés d'utilisation de ces systèmes d'enzymes afin de détecter l'acétone dans des échantillons biologiques ou prélevés dans l'environnement. L'invention porte aussi sur des biocapteurs contenant ces systèmes d'enzymes et permettant de procéder à la détection d'acétone par connexion d'un dispositif électrochimique, photométrique ou autre dispositif de détection à une ou plusieurs réactions ou un ou plusieurs canaux d'enzymes propres à l'acétone. L'invention concerne également des procédés d'utilisation de ces biocapteurs propres à l'acétone tels que le contrôle de la perte de poids chez un sujet, la détection de maladies, et la surveillance de la biodisponibilité des traitements.

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