Analytical kit

Details Analytical kit Analytical kit

C - Chemistry, Metallurgy

12

Q

C12Q 1/60 (2006.01) C12Q 1/28 (2006.01) G01N 33/52 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2207555

The invention relates to an analytical kit for determining a level of an analyte on a solid surface, comprising (a) a source of hydrogen peroxide; (b) a source of an affinity-enzymatic compound of formula A-C-B, wherein A is a detecting agent having affinity for the analyte, B is an enzymatic visualizing agent consisting of peroxidase and C is a binding agent linking the detecting agent and the visualizing agent to one another; and (c) a multilayer analytical element comprising a solid support and a hydrophilic layer thereon, the hydrophilic layer being formed of gelatin, polyvinyl alcohol or a mixture thereof and containing a developing agent and a hydrophobic color-producing agent in a total amount of 5 to 30 weight %, the developing agent and the color-producing agent being present in a weight ratio of 1:0.3 to 1:3Ø The developing agent and the color-producing agent co-act with one another in a manner such that, when a predetermined amount of the affinity-enzymatic compound is applied onto the surface in a selected area thereof and allowed to stand thereon for a period of time sufficient to cause binding of the detecting agent to the analyte on the surface, any affinity-enzymatic compound with unbound detecting agent is removed from the surface, a predetermined amount of hydrogen peroxide is thereafter applied onto the surface in the selected area and the the multilayer analytical element is pressed against the surface in the selected area, the developing agent undergoes oxidation by the hydrogen peroxide in the presence of the peroxidase bound via the binding and detecting agents to the analyte, and the developing agent in oxidized form -15- reacts with the color-producing agent to form a colored product having a color indicative of the level of the analyte. The analytical kit according to the invention can be used in medicine and pharmacology as well as in criminal law. It is preferably utilized for determining the level of cholesterol on human skin or other animal tissue. It can also be utilized for measuring chloride ions and glucose, thereby enabling the diagnosis of cysticfibrosis and diabetes.

Cette invention concerne un élément d'analyse multicouche destiné à effectuer des mesures quantitatives d'un composant d'un échantillon grâce à un agent réactif pouvant entraîner une interaction avec ledit échantillon. Cet élément d'analyse multicouche comprend un support solide recouvert d'une couche hydrophile constituée de gélatine, d'alcool polyvinylique ou d'un mélange des deux. Cette couche hydrophile contient également une substance révélatrice ainsi qu'un agent générateur de couleur dans un volume total représentant de 5 à 30 % en poids, la substance révélatrice et l'agent générateur de couleur étant présents dans un rapport en poids allant de 1:0,3 à 1:3,0. La substance révélatrice doit pouvoir être oxydée par l'agent réactif en présence d'un agent oxydant, ce qui lui permettra, sous sa forme oxydée, de réagir avec l'agent générateur de couleur pour former un produit coloré. L'élément d'analyse multicouche de cette invention peut être employé en médecine, en pharmacologie ainsi qu'en droit pénal. Il peut être utilisé pour mesurer les taux de cholestérol, d'ions chlorure et de glucose, permettant ainsi de diagnostiquer l'hypercholestérolémie, la mucoviscidose et les diabètes.

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