Analytical method and element for protein assay

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1312266

-i- ANALYTICAL METHOD AND ELEMENT FOR PROTEIN ASSAY Abstract of the Disclosure There is disclosed a method for quantita- tively determining protein, comprising the steps of: a) providing a sample of the protein in an aqueous medium; b) providing an aqueous medium having a pH in excess of 12 and comprising i) a cupric salt and a pyridyl-azo dye; or ii) a preformed cupric-pyridylazo dye complex; c) combining the aqueous mediums of a) and b) thereby providing a color having an intensity which is inversely proportional to the amount of unreacted dye present in the combined mediums; and d) determining the quantity of protein present in the sample colorimetrically. The method can be used with a multilayer dry analytical element.

549198

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