Anti-microbial agents, diagnostic reagents, and vaccines...

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/002 (2006.01) A61K 31/00 (2006.01) A61P 33/02 (2006.01) C07K 16/40 (2006.01) C12N 1/11 (2006.01) C12N 9/88 (2006.01) C12N 15/60 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2377131

This invention relates uses of components of plant-like metabolic pathways not including psbA or PPi phosphorfructokinase and not generally operative in animals or encoded by the plastid DNA, to develop compositions that interfere with Apicomplexan growth and survival. Components of the pathways include enzymes, transit peptides and nucleotide sequences encoding the enzymes and peptides, or promoters of these nucleotide sequences to which antibodies, antisense molecules and other inhibitors are directed. Diagnostic and therapeutic reagents and vaccines are developed based on the components and their inhibitors. A cDNA sequence that encodes chorismate synthase expressed at an early state of Apicomplexan development, is disclosed and may be altered to produce a "knockout" organism useful in vaccine production.

L'invention concerne des utilisations de constituants de voies métaboliques de type végétal, n'incluant pas la psbA ou PPi phosphofructokinase et généralement inopérants chez les animaux ou qui sont codés par l'ADN plastidial, pour le développement de compositions entravant la croissance et la survie du parasite Apicomplexan. Les constituants de ces voies incluent notamment des enzymes, des peptides de transit et des séquences nucléotidiques codant pour les enzymes et peptides, ou des promoteurs de ces séquences nucléotidiques contre lesquels sont dirigés des anticorps, des molécules anti-sens et d'autres inhibiteurs. L'invention concerne également des réactifs diagnostiques et thérapeutiques et des vaccins développés à base de ces constituants et de leurs inhibiteurs. L'invention concerne enfin une séquence d'ADNc codant pour la charismate synthase exprimée à un stade précoce du développement de l'Apicomplexan, que l'on peut modifier afin de produire un organisme inactivé, utile dans la production de vaccins.

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