Assay for paralytic shellfish toxin

G - Physics – 01 – N

Patent

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Details

G01N 33/566 (2006.01) C07K 14/81 (2006.01) G01N 1/34 (2006.01) G01N 21/78 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2430868

A method of detecting and/or measuring the amount of a paralytic shellfish toxin (PST) present in a sample, comprising the steps of: 1) providing an isolated and purified saxiphilin, or fragment thereof which contains a saxitoxin binding site; 2) contacting it with the sample; 3) measuring binding of PST contained in the sample to said isolated and purified saxiphilin; and correlating the amount of binding with either the presence or absence of PSTs in the sample or with the PST concentration in the sample.

L'invention concerne un procédé de détection et/ou de mesure, dans un échantillon, de la toxine paralysante présente dans des fruits de mer, ce procédé comprenant les étapes consistant: 1) à préparer une saxiphiline isolée et purifiée, ou un fragment de celle-ci contenant un site de liaison de saxitoxine, 2) à mettre en contact cette saxiphiline ou le fragment de celle-ci, avec l'échantillon, et 3) à mesurer la liaison de la toxine paralysante contenue dans l'échantillon avec la saxiphiline isolée et purifiée, et à corréler l'importance de cette liaison, soit avec la présence, soit avec l'absence de toxines paralysantes dans l'échantillon, soit avec la teneur de l'échantillon en toxine paralysante.

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