Assay method for detecting the presence of bacteria

Details Assay method for detecting the presence of bacteria Assay method for detecting the presence of bacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/04 (2006.01) C12Q 1/24 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2112603

An assay method is provided to easily and quickly detect the presence of organisms capable of being cultured, such as bacteria, characterized by antibody capture of the organism of interest by use of specialized magnetic beads, incubation of the captured cells to form colonies; removal of material from the colonies with a colony lift membrane; and detection of the colony material on the membrane sheet by use of labeled antibodies, PCR or nucleic acid probes.

Méthode de dosage permettant de détecter facilement et rapidement la présence d'organismes capables d'être cultivés, comme des bactéries; la méthode est caractérisée par la capture d'anticorps de l'organisme d'intérêt à l'aide de billes magnétiques spéciales, par l'incubation des cellules capturées en vue de former des colonies, par l'extraction du matériel des colonies à l'aide d'une membrane de transfert de colonies et par la détection du matériel de la colonie présent sur la membrane à l'aide d'anticorps étiquetés, de la PCR ou de sondes d'acide nucléique.

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