Assays for assembly of ebola virus nucleocapsids

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C12Q 1/18 (2006.01)

Patent

CA 2492068

The present invention relates to assays for the identification of compounds that inhibit assembly of NP, VP35, and VP24, or inhibit the glycosylation of NP, required for nucleocapsid formation, for use as anti-viral agents. The invention also relates to assays for the identification of compounds that block glycosylation of proteins having a glycosylation domain that is substantially homologous to a glycosylation domain of NP required for polymerization. The invention further relates to pseudoparticles for presentation of antigens or antigenic epitopes for immunogenic or vaccination purposes.

La présente invention concerne des dosages permettant d'identifier des composés inhibant le regroupement de NP, VP35, et de VP24, ou inhibant la glycosylation de NP, nécessaire dans la formation des nucléocapsides, utilisés comme agents antiviraux. L'invention concerne également des dosages permettant d'identifier des composés bloquant la glycosylation de protéines présentant un domaine de glycosylation sensiblement homologue à un domaine de glycosylation de NP nécessaire pour la polymérisation. L'invention concerne, de plus, des pseudoparticules de présentation des antigènes ou épitopes antigéniques à des fins immunogéniques ou de vaccination.

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