Assays utilizing improved chemiluminescent esters,...

C - Chemistry – Metallurgy – 09 – K

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31/104, 150/26,

C09K 11/07 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) C07D 219/06 (2006.01) C07D 221/12 (2006.01) C07D 401/12 (2006.01)

Patent

CA 1341613

This invention discloses specific binding assays which use a chemiluminescent compound which has increased stability in aqueous solution. The chemiluminescent moiety an ester, thiolester or amide in which the ester, thiolester or amide linkage is between a heterocyclic ring or ring system containing a carbon atom to which the linkage is attached and an aryl ring or ring system. Other chemiluminescent moieties are disclosed which are characterized by a heterocyclic ring or ring system and a secondary substituent of the formula R n X-, with the ester, thiolester or amide linkage being between the heterocyclic ring or ring system and a leaving group. Compositions including and assay kits incorporating such chemiluminescent moieties are also disclosed. These assays are not as susceptible to hydrolysis as is the prior art compounds and show increased stability in aqueous solution.

587320

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