Bacteria for expressing a polysaccharide depolymerase...

Details Bacteria for expressing a polysaccharide depolymerase... Bacteria for expressing a polysaccharide depolymerase...

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/56 (2006.01) C12N 1/20 (2006.01) C12N 9/24 (2006.01)

Patent

CA 1293210

ABSTRACT OF THE DISCLOSURE A segment of a bacteriophage encoding for a polysaccharide depolymerase which has been cloned and expressed in Escherichia coli is described. In particular, bacteriophage ERA103 was found to consist of five EcoRI fragments labeled: A, 7.5-kb; B, 5.0-kb; C, 2.7-kb, D, 2.1-kb and E, 1.8-kb. Fragment B encodes for the depolymerase and was cloned into the positive-selection vector pOP203(A2+), p8R322 and the expression vector pKK223-3. The depolymerase is applied to rosaceous plants to prevent Fireblight caused by Erwinia amylovora by depolymerizing a polysaccharide produced by this bacteria.

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