Biosensor

G - Physics – 01 – N

Patent

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G01N 27/327 (2006.01)

Patent

CA 2603123

It is intended to provide a sensor by which the concentration of neutral fat in a sample such as a biological sample can be quickly and highly accurately measured without resorting to a pretreatment of the sample. This object can be established by a biosensor having an electrode system, which comprises an insulating substrate and a working electrode and a counter electrode formed on the insulating substrate, and a reaction layer, which contains lipoprotein lipase, glycerol dehydrogenase and an electron acceptor and is formed in the upper part of the electrode system or close to it, whereby the neutral fat concentration is measured based on the current value flowing through the electrode system.

L~invention concerne une sonde par laquelle la concentration en graisse neutre dans un échantillon tel qu'un échantillon biologique peut être rapidement et très correctement mesurée sans avoir recours à un prétraitement de l~échantillon. L'invention concerne donc une biosonde ayant un système d'électrode qui comprend un substrat isolant et une électrode active et une contre-électrode formées sur le substrat isolant, et une couche réactive qui contient de la lipoprotéine lipase, de la glycérol déshydrogénase et un accepteur d'électron. Ladite biosonde est formée dans la partie supérieure du système d'électrode ou près de celle-ci, et la concentration en graisses neutres est mesurée en se basant sur la valeur actuelle s~écoulant à travers le système d'électrode.

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