Bzip transcription factor that controls expression of the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07H 21/04 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2394018

cDNAs (RISBZ1, RISBZ4, and RISBZ5) encoding bZIP transcription factors were isolated from a cDNA library originating in rice plant seed. The cDNAs encode novel proteins and have binding activity to the GCN4 motif. Among them, RISBZ1 activated transcription mediated by the GCN4 motif by 100-fold or more. Since the expression of RISBZ1 precedes the expression of a seed storage protein gene and is expressed only in maturing seeds, it is suggested that RISBZ1 controls the expression of rice seed storage proteins. In addition, by linking the recognition sequence of the transcription factor, the GCN4 motif, in tandem and introducing it into the promoter for a gene encoding seed storage protein to facilitate its binding to the transcription factor RISBZ1, expression of a foreign gene under the control of the modified promoters is greatly enhanced.

L'invention concerne l'isolation des ADNc (RISBZ1, RISBZ4 ET RISBZ5) codant pour les facteurs de transcription de type bZIP à partir d'une bibliothèque d'ADNc construite à partir du grain de riz. Ces ADNc codent pour de nouvelles protéines et présentent une activité de liaison au motif GCN4. RISBZ1 active la transcription 100 fois ou plus via le motif GCN4. L'expression de RISBZ1 précède l'expression d'un gène de protéine de stockage du grain et est observée exclusivement dans des graines au cours de l'étape de maturation, ce qui signifie que RISBZ1 régule l'expression de la protéine de stockage du grain de riz. Pour faciliter la liaison au facteur de transcription de RISBZ1, la séquence de reconnaissance (motif GGN4) du facteur de transcription est ligaturé en série et transféré dans un promoteur d'une protéine de stockage spécifique. Ainsi, l'expression d'un gène étranger par régulation du promoteur ainsi modifié peut être augmentée.

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