Cell death inducing agent

Details Cell death inducing agent Cell death inducing agent

C - Chemistry, Metallurgy

07

K

C07K 16/28 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) A61P 35/02 (2006.01) A61P 37/02 (2006.01) A61P 43/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 15/11 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2548929

A DNA expression vector encoding 2D7sc(Fv)2, wherein the variable heavy chain (VH) domain sequence and the variable light chain (VL) domain sequence of 2D7 antibody are linked together via 15mer linkers in the order of VH-VL-VH-VL, is constructed. Then this vector is transferred into CHO cells to thereby establish a 2D7sc(Fv)2 production expression cell line. 2D7sc(Fv)2 expressed in this cell line is purified and subjected to a cell death induction experiment. As a result, it is clarified that 2D7sc(Fv)2 has an activity of concentration-dependently inducing cell death.

L'invention concerne la construction d'un vecteur d'expression d'ADN codant pour 2D7sc (Fv)2, la séquence domaine chaîne lourde (VH) variable et la séquence domaine chaîne légère (VL) variable de l'anticorps 2D7 sont liées par des liens 15mer dans l'ordre VH-VL-VH-VL. Ce vecteur est ensuite transféré dans des cellules CHO pour établir une lignée cellulaire d'expression de production 2D7sc (Fv) 2. 2D7sc (Fv)2 exprimé dans la lignée cellulaire est purifié et soumis à une expérimentation d'induction de mort cellulaire. Il s'avère que 2D7sc(Fv)2 a, en fonction de la concentration, une activité d'induction de la mort cellulaire.

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