Cellular assay method for identifying pkc-0 inhibitors

Details Cellular assay method for identifying pkc-0 inhibitors Cellular assay method for identifying pkc-0 inhibitors

G - Physics

01

N

G01N 33/50 (2006.01) C07K 16/40 (2006.01) C12Q 1/48 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2609640

The invention relates to a method for investigating the modulating effect of a test substance on a PKC.theta.-dependent signal transduction pathway or for finding a PKC.theta. modulator in a human or animal cell, including the steps (a) contacting the cell with the test substance or with the PKC.theta. modulator ; (b) where appropriate inducing the kinase activity of PKC.theta. ; (c) incubating the cell under conditions which bring about phosphorylation at least of a serine or threonine residue of PKC.theta. ; (d) where appropriate lysing the cell ; and (e) determining the phosphorylation content of the at least one serine or threonine residue of PKC.theta..

L'invention porte sur une méthode visant à étudier l'effet de modulation d'une substance test sur un mécanisme d'action de transduction du signal dépendant de PKC? ou rechercher un modulateur de PKC? dans une cellule humaine ou animale, cette méthode consistant à: (a) mettre en contact la cellule avec la substance test ou avec le modulateur de PKC?; (b) si approprié, induire l'activité de la kinase de PKC?; (c) incuber la cellule dans des conditions qui amènent à la phosphorylation d'au moins un résidu de sérine ou thréonine de PKC?; (d) si approprié, lyser la cellule; et (e) déterminer la proportion de la phosphorylation d'au moins un résidu de sérine ou thréonine de PKC?.

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