Cellulase cel5h related reagents and their use in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/42 (2006.01) C12N 15/62 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2744607

The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterises a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerisation of cellulose containing substrates.

La présente invention concerne des applications de la cellulase Cel5H de Saccharophagus degradans et ses homologues, fragments fonctionnels et/ou variants et formes modifiées de celle-ci, dans le contexte de micro-organismes recombinants, plus particulièrement solvantogènes, plus particulièrement C. acetobutylicum. Linvention concerne en outre un nouveau domaine de la cellulase Cel5H avec une fonction de module de liaison de cellulose putative, et ses utilisations dans des protéines chimériques pour la dépolymérisation de substrats contenant de la cellulose.

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