Chamber with adjustable volume for cell culture and organ...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/04 (2006.01)

Patent

CA 2471706

The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g.,liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.

L'invention concerne des chambres modulaires pour culture cellulaire, à volume adaptable, mais sans perte d'étanchéité ou de stérilité. Le principe consiste à régler ledit volume, c'est-à-dire le volume compris entre deux plaques enserrant un joint compressible et une butée sensiblement incompressible, au moyen d'un joint étanche entre les plaques, selon une pluralité de niveaux de compression. On peut pratiquer la culture cellulaire entre des plaques sensiblement parallèles et rigides, en ayant la possibilité d'utiliser un volume important pour l'ensemencement des cellules et de réduire le volume de rétention pour la perfusion, sans ouvrir ou démonter le système de quelque manière que ce soit et porter atteinte à l'intégrité de l'étanchéité et de la stérilité. Cette chambre de culture cellulaire, fermée, modulaire, à volume adaptable, peut donc être perfusée et utilisée pour la culture cellulaire (par exemple, hépatocytes), en offrant des niveaux élevés de fonction cellulaire dans les systèmes d'assistance d'organe (par exemple, foie), aux fins des applications suivantes: systèmes de production de cellules, ou de production de produits dérivés de cellules, du type protéines et virus, ou encore systèmes de traitement de liquides biologiques pour l'élimination de toxines, du type ammoniac, ou d'adjonction de produits de synthèse cellulaire, voire les deux à la fois.

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