Cho /cert cell lines

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/67 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2759602

The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).

L'invention concerne le domaine de la technologie des cultures cellulaires. L'invention décrit des lignées de cellules hôtes de production comprenant des produits de construction vecteurs comprenant une cassette d'expression CERT S132 A. Ces lignées cellulaires ont des caractéristiques de croissance améliorées et des taux d'expression de CERT S132 A élevés. L'invention concerne notamment deux lignées cellulaires déposées auprès du DSMZ sous le numéro DSM ACC2989 (CHO/CERT 2.20) et DSM ACC2990 (CHO/CERT 2.41). L'invention porte en outre sur un procédé de génération de telles cellules hôtes de production privilégiées et sur un procédé de production de protéines à l'aide des deux lignées cellulaires déposées auprès du DSMZ sous le numéro DSM ACC2989 (CHO/CERT 2.20) et DSM ACC2990 (CHO/CERT 2.41).

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