C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.18, 195/1.
C12N 15/12 (2006.01) C07H 21/04 (2006.01) C12N 5/08 (2006.01) C12N 9/48 (2006.01) C12N 9/72 (2006.01)
Patent
CA 1295959
ABSTRACT OF THE DISCLOSURE The invention provides a chromosomal DNA sequence encoding human tissue plasminogen activating factor free of the SmaI recognition site between the 8th and the 9th exon; an expression vector which has a DNA sequence comprising a chromosomal DNA sequence coding for human tissue plasminogen activating factor and a promoter region functioning in cultured cells derived from a gene other than human tissue plasminogen activat- ing factor as joined to said chromosomal DNA sequence; cultured cells transfected with such expression vector for human tissue plasminogen activating factor; and a method of producing human tissue plasminogen activating factor which comprises cultivating cultured cells transfected with such expression vector for human tissue plasminogen activating factor and isolating the human tissue plasminogen activating factor produced by the cells.
513426
Kakutani Tetsu
Kawaharada Hajime
Maruyama Hiroyuki
Matsumoto Keiji
Watanabe Kiyoshi
Borden Ladner Gervais Llp
Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
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