Cloning and utilization of aminotransferase genes

Details Cloning and utilization of aminotransferase genes Cloning and utilization of aminotransferase genes

C - Chemistry, Metallurgy

12

N

195/1.22

C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12P 13/04 (2006.01)

Patent

CA 1341352

This invention relates to a method of increasing the rate of transamination of a susceptible substrate by the gene product of the aspC, tyrB or ilvE gene comprising: a) inserting a plasmid containing aspC, tyrB or ilvE gene into a microorganism; b) expressing the aspC, tyrB or ilvE gene in the microorganism resulting in the synthesis of an active aminotransferase; c) catalyzing the transamination of the susceptible substrate by the aminotransferase. The method is useful, for example, where the substrate is phenylpyruvic acid and the amino acid is L-phenylalanine.

616140

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