C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12Q 1/04 (2006.01)
Patent
CA 2419254
The present invention relates generally to compositions and methods for detecting target microorganisms in a sample. In particular, the present invention relates to compositions comprising a conditionally detectable maker and an enzyme substrate that provides detection of the target microorganism as well as methods of use thereof. With the invention disclosed herein, a widely applicable approach is revealed which utilizes a presumptive indicator for the target microorganism and non-target microorganisms capable of growth and detection in a medium followed by a confirmation indicator, which is substantially only reacted upon by the non-target microorganisms.
De façon générale, la présente invention concerne des compositions et des méthodes permettant de détecter des micro-organismes cibles dans un échantillon et, en particulier, des compositions qui renferment un marqueur détectable sous certaines conditions et un substrat enzymatique pour la détection du micro-organimse cible. Cette invention porte sur une méthode au large champ d'application qui fait intervenir un indicateur présumé pour le micro-organisme cible et pour des micro-organismes non cibles capables de croissance et pour leur détection dans un milieu, puis un indicateur de confirmation auquel pour l'essentiel seuls les micro-organismes non cibles réagissent.
Biocontrol Systems Inc.
Gowling Lafleur Henderson Llp
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