Compositions and methods for nucleic acid extraction from...

Details Compositions and methods for nucleic acid extraction from... Compositions and methods for nucleic acid extraction from...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 1/08 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2509378

Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.

La présente invention concerne des procédés et des compositions permettant d'extraire des acides nucléiques d'un prélèvement biologique. Les compositions d'extraction contiennent une enzyme protéase telle que la protéinase K, à un pH alcalin avec peu ou pas de tensio-actif. L'extraction peut être effectuée de manière efficace en 60 minutes, voire moins, à température ambiante pour certains prélèvements de tissus mammaliens et à des températures élevées pour certains tissus végétaux.

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