C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 9/08 (2006.01) C12N 9/50 (2006.01) C12N 9/88 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2237120
DNA constructs, Pichia methanolica cells, and methods for producing polypeptides using the DNA constructs and cells are disclosed. The DNA constructs comprise a transcription promoter of a Pichia methanolica gene, a DNA segment encoding a polypeptide heterologous to P. methanolica, a transcription terminator of a P. methanolica gene, and a selectable marker. P. methanolica cells containing the DNA constructs are used within methods for producing polypeptides heterologous to P. methanolica. DNA molecules comprising transcription promoters of P. methanolica genes are also disclosed.
Constructions d'ADN, cellules de Pichia methanolica et procédés de production de polypeptides mettant en oeuvre de telles constructions d'ADN et de telles cellules. Les constructions d'ADN comprennent un promoteur de transcription du gène de Pichia methanolica, un segment d'ADN codant un polypeptide hétérologue par rapport à Pichia methanolica, une terminaison de transcription du gène de Pichia methanolica et un marqueur sélectionnable. Des cellules de Pichia methanolica renfermant des constructions d'ADN sont utilisées dans le cadre de ces procédés de production de polypeptides hétérologues par rapport à Pichia methanolica. Des molécules d'ADN renfermant des promoteurs de transcription des gènes de Pichia methanolica sont également visées par l'invention.
Fetherstonhaugh & Co.
Zymogenetics Inc.
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