Constructing and screening a dna library of interest in...

Details Constructing and screening a dna library of interest in... Constructing and screening a dna library of interest in...

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 9/00 (2006.01) C12N 9/20 (2006.01) C12N 15/10 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2344619

A method of constructing and screening a library of polynucleotide sequences of interest in filamentous fungal cells by use of an episomal replicating AMA1- based plasmid vector, thus achieving a high frequency of transformation and a stable and standard uniformly high level of gene expression.

L'invention concerne un procédé d'établissement et de criblage d'une banque de séquences polynucléotidiques d'intérêt dans des cellules fongiques filamenteuses au moyen d'un vecteur plasmidique de reproduction AMA1 épisomique, afin d'obtenir une fréquence élevée de transformation et un haut niveau stable et standard d'expression génique.

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