Control of gene expression in eukaryotic cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A61K 31/34 (2006.01) A61K 31/365 (2006.01) A61K 48/00 (2006.01) C07D 307/33 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2377932

DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter ("lux box") or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.

L'invention concerne des produits de recombinaison d'ADN, ainsi que d'autres compositions et méthodes de régulation de l'expression génique dans des cellules et organismes eucaryotes, ces produits de recombinaison étant dérivés de systèmes de détection de quorums bactériens. Un ou plusieurs éléments cis provenant du promoteur luxI (<= boîte lux >=) ou une séquence fonctionnellement similaire sont incorporés dans un promoteur eucaryote. Une protéine réceptrice de la famille LuxR de régulateurs de transcription, après liaison avec un composé de lactone d'homosérine acylé (AHL), entre en interaction avec la boîte lux, modulant ainsi l'activité du promoteur.

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