C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01)
Patent
CA 2375111
The present invention refers to a differential and selective culture medium, for the detection of yeasts of the species <i>Zygosaccharomyces bailii</i> and <i>Zygosaccharomyces bisporus</i>, allowing a drastic reduction in the time and work usually involved in the conventional detection of these species. According to the present invention, the detection of <i>Zygosaccharomyces bailii</i> and <i>Zygosaccharomyces bisporus</i> is accomplished with one single test that only requires the preparation, and inoculation of one liquid or solid culture medium. This culture medium is comprised by a base mineral medium supplemented with oligoelements and vitamins, by glucose and formic acid as the only energy and carbon sources, and by an acid-base indicator. The acid-base indicator, particularly bromocresol green, provides the medium with a green coloring that is converted into blue through the action of the above mentioned yeasts. Additionally, the blue color presented by the coloniesis a specific characteristic of these species and can be observed in the medium after 48 to 96 hours of incubation, depending upon the inoculation methodology used. The invention can be used either with previously isolated and purified yeast strains or with cell suspensions of mixed yeast populations containing other yeasts different from <i>Zygosaccharomyces bailii</i> and <i>Zygosaccharomyces bisporus</i>, for the detection of these species in the food industry, namely in wines and other beverages. The medium can also be included in galleries of yeast identification tests.
L'invention porte sur un milieu de culture différentiel et sélectif pour la détection de levures des types Zygosaccharomyces bailii et Zygosaccharomyces bisporus, réduisant considérablement le temps et le travail usuellement nécessaire à cette détection. L'invention permet de détecter les Zygosaccharomyces bailii et Zygosaccharomyces bisporus en un seul essai ne nécessitant que la préparation et l'inoculation d'un milieu de culture liquide ou solide. Ledit milieu consiste en un milieu minéral de base additionné d'oligo-éléments et de vitamines, de glucose et d'acide formique, seules sources d'énergie et de carbone, et d'un indicateur de pH, du vert de bromocrésol colorant le milieu en vert et virant au bleu sous l'action des susdites levures. La coloration en bleu, spécifique de ces colonies s'observe dans le milieu après 48 à 96 heures d'incubation, selon la méthode d'inoculation utilisée. L'invention peut s'utiliser avec des souches de levures préalablement isolées et purifiées, ou avec des suspensions de populations mixtes de levures comportant d'autres levures que les Zygosaccharomyces bailii et Zygosaccharomyces bisporus, en vue de leur détection dans l'industrie alimentaire, notamment dans les vins et autres boissons. Le milieu peut également être intégré à des galeries d'essais d'identification de levures.
Corte-Real Manuela
Leao Cecilia
Schuller Dorit
Corte-Real Manuela
Leao Cecilia
Schuller Dorit
Stab-Tratamento de Aguas E. Biotecnologia Lda.
Universidade Do Minho
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