C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A23L 1/227 (2006.01) A23L 1/236 (2006.01) C07K 14/415 (2006.01) C07K 14/43 (2006.01) C12N 15/10 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2062174
CURCULIN B AND DNA ENCODING SAME, AND PROCESS FOR PRODUCTION THEREOF ABSTRACT OF THE DISCLOSURE Essentially pure curculin B. A process for producing curculin B, comprising: culturing a transformed cell or microorganism containing a recombinant DNA containing a base sequence encoding curculin B, whereby curculin B is produced by said transformed cell or microorganism, and isolating curculin B from said transformed cell or microorganism. A DNA encoding mature or premature curculin B. A process for producing a DNA including a base sequence encoding curculin B, comprising: separating a fraction containing a curculin B mRNA from Curculigo latifolia, preparing a single-stranded DNA from said mRNA using reverse transcriptase, preparing a double-stranded DNA from said single-stranded DNA, inserting said double-stranded DNA into a vector, transforming a host with said vector to produce a cDNA library, and isolating a cDNA encoding curculin B from said library, using one or more synthesized DNA's containing a base sequence coding for a partial amino acid sequence elucidated from curculin A purified from Curculigo latifolia, as a probe.
Abe Keiko
Arai Soichi
Kurihara Yoshie
Yamashita Haruyuki
Arai Soichi
Kurihara Yoshie
Mcfadden Fincham
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