Delivery of macromolecules into cells

Details Delivery of macromolecules into cells Delivery of macromolecules into cells

A - Human Necessities

61

N

A61N 1/30 (2006.01) A61N 1/32 (2006.01)

Patent

CA 2372409

An object of the invention is to provide a method for delivery of macromolecules into biological cells, such as Langerhans cells (22) in the epidermis (20) of a patient, which includes the steps of coating electodes (16) in an electrode assembly (12) with solid phase macromolecules to be delivered, such as a DNA, and/or RNA vaccine or a protein- based vaccine, attaching the electrode assembly (12) having the coated electrodes (16) to an electrode assembly holder (13), providing a waveform generator (15), establishing electrically conductive pathways between the electrodes (16), and the waveform generator (15), locating the electrodes (16) such that the biological cells are situated therebetween, such as by penetrating the needle electrode (16) into the epidermis (20) above the epidermal basal lamina, and providing pulse waveform from the waveform generator (15) to the electrodes (16), such that macromolecule on the electrodes (16) is driven off of the electrodes (16), and delivered into the biological cells, such as the Langerhans cells (22).

L'invention concerne un procédé d'administration de macromolécules dans des cellules biologiques, notamment les cellules de Langerhans (22) formant l'épiderme (20) d'un patient. Ce procédé consiste à revêtir les électrodes (16) d'un ensemble d'électrodes (12) avec une macromolécule en phase solide à administrer, telle qu'un vaccin AND et/ou ARN ou un vaccin protéique, à fixer l'ensemble d'électrodes (12) contenant les électrodes revêtues (16) à un support (13), à apporter un générateur d'ondes (15), à établir des voies conductrices d'électrcité entre les électrodes (16) et le générateur d'ondes (15), à placer les électrodes (16) de manière à intercaler les cellules biologiques par introduction d'une électrode aiguille (16) dans l'épiderme (20) au-dessus de la lame ventrale épidermique et à apporter une onde d'impulsion provenant du générateur (15) vers les électrodes (16) de sorte que la macromolécule sur les électrodes (16) soit entraînée loin des électrodes (16) et administrée dans les cellules biologiques, notamment les cellules de Langerhans.

Affiliated with

Also associated with

Rating

Delivery of macromolecules into cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Delivery of macromolecules into cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Delivery of macromolecules into cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1539112