Detection and biodegradation of explosives

Details Detection and biodegradation of explosives Detection and biodegradation of explosives

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) A62D 3/02 (2007.01) C12S 99/00 (2010.01) B09C 1/10 (2006.01) C02F 3/34 (2006.01) C12N 1/20 (2006.01) C12N 9/06 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/26 (2006.01) G01N 33/22 (2006.01)

Patent

CA 2226729

An enzyme capable of catalyzing the removal of nitrite from pentaerythritol tetranitrate (PETN) is provided. The enzyme (known as PETN reductase enzyme) is produced by culturing a novel strain of the Enterobacter cloacae bacterium isolated from nature. The strain designated PB2 has been deposited as NCIMB 40718. The amino acid sequence of the enzyme and the genetic sequence which encodes for this enzyme have also been determined. A PETN reductase enzyme encoded by the on gene is provided. A method for producing PETN reductase enzyme in large quantities and methods of bioremediation using the enzyme so produced are also provided. Additionally there is provided a method of detecting the presence of PETN in a sample together with a biosensor for use in such a method.

L'invention concerne une enzyme capable de catalyser la suppression du nitrite contenu dans du pentaérythritol tétranitrate (PETN). On obtient cette enzyme (appelée PETN réductase) au moyen de la culture d'une nouvelle souche d'Enterobacter cloacae bacterium isolée du milieu naturel. On a déposé cette souche désignée PB2 en tant que NCIMB 40718. On a également déterminé la séquence d'acides aminés de l'enzyme, ainsi que la séquence génétique codant pour cette enzyme. L'invention concerne une enzyme PETN réductase codée par le gène onr. Elle concerne également un procédé de production de l'enzyme PETN réductase en quantités importantes, ainsi que des procédés de biodégradation accélérée au moyen de l'enzyme obtenue. Elle concerne, de plus, un procédé servant à détecter la présence de PETN dans un spécimen, ainsi qu'un biodétecteur servant à mettre ce procédé en application.

Affiliated with

Also associated with

Rating

Detection and biodegradation of explosives does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection and biodegradation of explosives, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection and biodegradation of explosives will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1955925