Detection assays and use thereof

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2730565

The invention provides compositions and methods for the detection and/or quantification of biological targets (e.g., nucleic acids and proteins) by the nucleic acid-templated creation of one or more reaction products, for example, epitopes, enzyme substrates, enzyme activators, and ligands. The reaction products can be detected and/or quantitated after signal amplification using an amplification system.

L'invention concerne des compositions et des procédés pour la détection et/ou la quantification de cibles biologiques (par exemple des acides nucléiques et des protéines) par création basée sur un modèle d'acide nucléique d'un ou plusieurs produits réactionnels, par exemple des épitopes, des substrats d'enzyme, des activateurs d'enzyme, et des ligands. Les produits réactionnels peuvent être détectés et/ou quantifiés après amplification de signal en utilisant un système d'amplification.

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