Detection instrument with the use of polynucleotides mapped...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C07K 14/415 (2006.01) C12M 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2562721

It is intended to provide an instrument for detecting gene expression, an instrument for detecting a gene polymorphism, an instrument for detecting an expressed protein and an instrument for detecting a substance interacting with an expressed protein which are usable in barley breeding. Polynucleotides comprising gene marker sequences and EST sequences mapped on barley chromosome, proteins encoded by the EST sequences and antibodies against these proteins are immobilized on a support. Further, the polynucleotides etc. immobilized on the support are aligned in the order as on the barley chromosome.

Il est prévu de fournir un instrument destiné à la détection de l'expression d'un gène, un instrument destiné à détecter un polymorphisme de gène, un instrument destiné à détecter une protéine exprimée et un instrument destiné à détecter une substance interagissant avec une protéine exprimée, lesquels peuvent être utilisés dans la culture de l'orge. Des polynucléotides comprenant des séquences de marqueurs de gène et des séquences EST mappées sur un chromosome d'orge, des protéines codées par les séquences EST et des anticorps contre ces protéines sont immobilisés sur un support. En outre, les polynucléotides, etc., immobilisés sur le support, sont alignés selon un ordre identique à celui du chromosome de l'orge.

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