Detection of antibiotic-resistant microorganisms

Details Detection of antibiotic-resistant microorganisms Detection of antibiotic-resistant microorganisms

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2709519

Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin- sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.

L'invention concerne des procédés de détection de S. aureus résistant à la méthicilline (MRSA) et de S. aureus sensible à la méthicilline (MSSA) dans des tests de coamplification d'acides nucléiques. L'invention réduit avantageusement l'incidence de faux positifs lors de la détermination de MRSA pendant des tests en temps réel en exigeant la satisfaction d'un critère seuil qui exclut certaines co-infections de la détermination de MRSA. L'invention concerne en outre la détermination de MSSA, même lorsque MSSA est présent en association avec des bactéries résistantes à la méthicilline et négatives à la coagulase (MR-CoNS) à des concentrations élevées ou faibles.

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