Detection of fermentation-related microorganisms

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2322780

Unique DNA sequences are provided which are useful in identifying different fermentation-related microorganisms, such as those involved in fermentations. These unique DNA sequences can be used to provide oligonucleotide primers in PCR based analysis for the identification of fermentation-related microorganisms. The DNA sequences of the present invention include the internal transcribed spacer (ITS) of the ribosomal RNA gene regions of particular fermentation-related microorganisms, as well as oligonucleotide primers which are derived from these regions which are capable of identifying the particular microorganism.

L'invention concerne des séquences d'ADN uniques qui servent à identifier différents micro-organismes liés à la fermentation, par exemple ceux qui participent à des processus de fermentation. On peut utiliser ces séquences d'ADN uniques pour fournir des amorces polynucléotidiques pour l'analyse basée sur ACP et visant à détecter des micro-organismes liés à la fermentation. Les séquences d'ADN de la présente invention comprennent l'espaceur interne transcrit des régions géniques de l'ARN ribosomique de micro-organismes déterminés liés à la fermentation ainsi que des amorces oligonucléotidiques dérivées de ces régions et capable d'identifier un micro-organisme déterminé.

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