Detection of hydrolyzing enzymes

C - Chemistry – Metallurgy – 12 – Q

Patent

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150/15.2, 150/3.

C12Q 1/34 (2006.01) C12Q 1/40 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1294194

DETECTION OF HYDROLYZING ENZYMES Abstract of the Disclosure A composition of a polysaccharide with a detectable material appended thereto and a 1,3-dione having a methylene group between the two carbonyls of the 1,3-dione, which methylene group has at least one ionizable hydrogen, provides accurate results in assays of hydrolyzing enzymes such as amylase. The composition can be incorporated in the reagent zone of a dry test element having a reagent zone and a registration zone.

525849

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