Detection of prostate cancer using psa glycosylation patterns

G - Physics – 01 – N

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G01N 33/574 (2006.01) C07K 16/30 (2006.01)

Patent

CA 2731823

The present invention features novel methods for determining if a subject has prostate caner. The present invention is based on the development of lectin immunosorbant assays which analyze .alpha.2,6-linked sialylation of total serum PSA by sambu-cus nigra lectin (SNA) and .alpha.2,3 -linked sialylation of total and free serum PSA. These novel assays were used then to conduct a clinical investigation of the potential role of glycoprotein analysis in improving PSA's cancer specificity. The present invention also features kits for determining if a subject has prostate cancer comprising one or more lectins and a PSA specific antibody and instructions for use.

La présente invention porte sur de nouveaux procédés de détermination de ce qu'un sujet a un cancer de la prostate. La présente invention porte sur le développement d'essais par immunoadsorbant de lectine qui analyse une sialylation liée à a2,6 d'antigène prostatique spécifique (APS) de sérum total par lectine de sambucus nigra (SNA) et sialylation liée à a2,3 d'APS dans le sérum total et libre. Ces nouveaux essais ont été utilisés pour mener une recherche clinique sur le rôle potentiel d'une analyse de glycoprotéine dans l'amélioration de la spécificité du cancer de l'APS. La présente invention porte également sur des ensembles destinés à déterminer si un sujet a un cancer de la prostate, comprenant une ou plusieurs lectines et un anticorps spécifique de l'APS et des instructions d'utilisation.

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