Devices and methods of cell capture and analysis

G - Physics – 01 – N

Patent

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G01N 33/53 (2006.01) C12Q 1/02 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2756493

The present invention provides a device for isolating target biomolecules or cells from samples, particularly bio-logical samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding en-tity that specifically binds to the target biomolecule or target cell; and a micro- channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.

La présente invention concerne un dispositif pour isoler des molécules biologiques ou des cellules cibles à partir d'échantillons, en particulier des échantillons biologiques. En particulier, le dispositif comprend un mélange de charge, qui contient l'échantillon biologique et une première entité de liaison qui se lie spécifiquement à la molécule biologique cible ou à la cellule cible, et un microcanal revêtu d'une seconde entité de liaison qui se lie directement ou indirectement à la première entité de liaison. L'invention concerne également des procédés de capture, de détection et/ou d'évaluation de molécules biologiques cibles ou de cellules cibles (par exemple, des cellules cancéreuses) dans des échantillons biologiques.

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