Dna mini-circles and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2732212

Methods and kits for generating circular nucleic acids in a cell-free system, and uses for the generated circular nu-cleic acids are provided. The methods comprise in vitro amplification of a nucleic acid template comprising a recombination site to produce tandem repeat nucleic acid sequence, and employ a recombination protein to generate the circular nucleic acids from the tandem repeat nucleic acid sequence.

L'invention porte sur des procédés et des kits pour générer des acides nucléiques circulaires dans un système exempt de cellule, et sur des utilisations pour les acides nucléiques circulaires générés. Les procédés comportent une amplification in vitro d'un modèle d'acide nucléique comportant un site de recombinaison afin de produire une séquence d'acide nucléique répétée en tandem, et emploient une protéine de recombinaison afin de générer les acides nucléiques circulaires à partir de la séquence d'acide nucléique répétée en tandem.

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