C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12P 19/18 (2006.01)
Patent
CA 2190149
The invention relates to DNA sequences coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear .alpha.- 1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems. The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity. The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.
Séquences d'ADN codant des protéines possédant l'activité enzymatique d'une amylosucrase, ce qui permet à des bactéries, à des champignons ou à des systèmes sans cellules d'effectuer la synthèse d'.alpha.-1,4 glycannes linéaires, à partir de sucrose du substrat. L'invention concerne également des plasmides ainsi que des bactéries contenant ces séquences d'ADN, de même que des procédés de production de plantes et de micro-organismes capables d'exprimer de manière intra ou extracellulaire un polypeptide possédant une activité amylosucrase. L'invention concerne encore la production de fructose pur par utilisation de protéines démontrant l'activité enzymatique d'amylosucrase.
Buttcher Volker
Koßmann Jens
Welsh Thomas
Bayer Bioscience Gmbh
Fetherstonhaugh & Co.
Institut Fur Genbiologische Forschung Berlin Gmbh
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