Drug targets in candida albicans

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 31/70 (2006.01) A61K 38/16 (2006.01) C07K 14/40 (2006.01) C07K 16/14 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2339337

The present invention is concerned with a method of identifying compounds which selectively modulate expression of polypeptides which are crucial for growth and survival of Candida albicans, which method comprises: (a) contacting a compound to be tested with one or more Candida albicans cells having a mutation in a nucleic acid molecule corresponding to the sequences according to any of claims 1 to 8 which mutation results in overexpression or underexpression of said polypeptides, in addition to contacting one or more wild type Candida albicans cells with said compound, (b) monitoring the growth and/or activity of said mutated cell compared to said wild type; wherein differential growth or activity of said one or more mutated Candida cells is indicative of selective action of said compound on a polypeptide or another polypeptide in the same or a parallel pathway. Also disclosed in the present invention are compounds identified and the sequences themselves which are critical for survival and growth of Candida albicans.

L'invention porte sur un procédé d'identification de composés modulant sélectivement l'expression de polypeptides essentiels pour la croissance et la survie du Candida albicans. Ladite méthode consiste: (a) à mettre en contact le composé à tester avec une ou plusieurs cellules de Candida albicans dont une molécule d'acide nucléique présente une mutation correspondant aux séquences des revendications 1 à 8, ladite mutation se traduisant par une surexpression ou une sousexpression desdits polypeptides, et ceci après avoir mis en contact une ou plusieurs cellules de Candida albicans de type sauvage avec ledit composé; et (b) à suivre la croissance et/ou l'activité de ladite cellule mutée par rapport à celle de la cellule de type sauvage, la différence de croissance ou d'activité de la ou des cellules de Candida albicans indiquant l'action sélective dudit composé sur ce polypeptide (ou un autre) selon un mode d'action identique ou parallèle. L'invention porte également sur les composés identifiés et les séquences qui sont critiques pour la survie et la croissance du Candida albicans.

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