Dry creatinine analytical element and method for its use

Details Dry creatinine analytical element and method for its use Dry creatinine analytical element and method for its use

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/26 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2017524

-0- DRY CREATININE ANALYTICAL ELEMENT AND METHOD FOR ITS USE Abstract of the Disclosure Herein disclosed is a multilayer analytical element comprising a support having thereon in the following order: a) a first reagent layer comprising sarcosine oxidase, a rate limiting amount of creatinine amidohydrolase; a peroxidative substance and imidazole leuco dye which is capable providing a detectable dye in the presence of said peroxidative substances and hydrogen peroxide; b) a second reagent layer containing creatinine amidinohydrolase; and c) a porous spreading layer; characterized in that a high NEG oxidase activity sarcosine oxidase having an activity of at least 300 I.U./m2 relative to N-ethyl glycine is present in i) the porous spreading layer; ii) a separate layer between the spreading layer and the layer containing creatine amidinohydrolase; iii) the layer containing creatine amidinohydrolase or iv) all of such layers i) - iii).

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