Dry powder formulation for protein labeling

Details Dry powder formulation for protein labeling Dry powder formulation for protein labeling

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2420149

The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a dry powdered mixture containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample are heated together and held at an elevated temperature until some or all of the powder dissolves.

La présente invention permet de coupler avec une efficacité exceptionnelle à un colorant les protéines d'un prélèvement biologique dont on cherche à analyser la composition protéinique par chromatographie, en combinant ledit prélèvement avec un mélange de poudre sèche contenant le colorant, un agent tampon et, dans des modes de réalisation préférés, un agent dénaturant également. La forme de poudre sèche du mélange permet d'éviter la détérioration ou la décomposition du colorant, et la combinaison des composants du mélange permet au colorant de se coupler de manière relativement uniforme et hautement contrôlable aux protéines lorsque l'on chauffe, ensemble, le mélange en poudre et le prélèvement et qu'on les maintient à température élevée jusqu'à ce que la poudre se dissolve totalement ou en partie.

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