Ecl labels having improved non-specific binding properties,...

Details Ecl labels having improved non-specific binding properties,... Ecl labels having improved non-specific binding properties,...

C - Chemistry, Metallurgy

07

D

C07D 213/22 (2006.01) C07D 213/30 (2006.01) C07D 213/34 (2006.01) C07D 213/40 (2006.01) C07D 213/81 (2006.01) C07D 471/04 (2006.01) C07F 9/58 (2006.01) C07F 9/6561 (2006.01) C07F 15/00 (2006.01) C07K 1/13 (2006.01) C12Q 1/68 (2006.01) G01N 33/532 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2451962

Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non- specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.

L'invention concerne des ligands de bipyridine ou de phénanthroline possédant des groupes fonctionnels qui empêchent les liaisons non spécifiques (plus particulièrement, des groupes fonctionnels chargés négativement qui ne sont pas affectés par des conditions normales pour conjuguer des réactifs biologiques à travers des liaisons amide), ainsi que des complexes de métal luminescents contenant ces ligands. L'utilisation de complexes d'osmium et de ruthénium luminescents qui contiennent ces ligands dans des analyses par électrochimiluminescence montre que l'utilisation de ces marqueurs peut réduire considérablement la quantité de liaisons non spécifiques observées par rapport à des analyses effectuées à l'aide de réactifs marqués avec des marqueurs analogues qui ne possèdent pas de groupes fonctionnels diminuant les liaisons non spécifiques.

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