Enhancing accumulation of heterologous polypeptides in plant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/47 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2543605

The present invention relates to improved methods for increasing accumulation levels of heterologous proteins in plant seeds for molecular farming through targeted suppression of endogenous proteins that compete against the heterologous protein for limited resources, such as amino acids, ribosomal binding sites and portfolio of enzymes participating in translation and post- translational processing. Provided are methods to suppress expression of transcription factors in barley aquired for transcriptional activation of hordein genes, using either antisense "inhibition" or double stranded RNA- induced RNA interferance or post transcriptional gene silencing (PTGS).

La présente invention concerne des techniques améliorées permettant d'augmenter l'accumulation des niveaux de protéines hétérologues dans des graines de plante de façon à effectuer une culture moléculaire par suppression ciblée de protéines endogènes qui sont en concurrence avec la protéine hétérologue sur des ressources limitées, telles que des acides aminés, des sites de liaison ribosomaux et un portefeuille d'enzymes participant au traitement de traduction et de post-traduction. Cette invention concerne des techniques permettant de supprimer l'expression de facteurs de transcription dans de l'orge acquise en vue d'une activation transcriptionnelle des gènes d'orge soi, via l'inhibition antisens, soi via l'interférence ARN induite par ARN soi via le silençage de gènes post-transcriptionnels (PTGS).

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