Enzymatic ligation-based identification of nucleotide sequences

Details Enzymatic ligation-based identification of nucleotide sequences Enzymatic ligation-based identification of nucleotide sequences

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2476842

Disclosed herein are novel methods for use in the analysis of nucleic acids. Uses disclosed include polynucleotide expression analysis, detection of single nucleotide polymorphisms, detection of pathogens, and detection of genetically modified cells and organisms. The method can be practiced using purified preparations of nucleic acids or unpurified cell lysates. Also provided are diagnostic methods and kits for conducting the disclosed methods.

L'invention concerne de nouvelles méthodes d'analyse d'acides nucléiques. Ces méthodes comprennent l'analyse de l'expression d'un polynucléotide, la détection de polymorphismes d'un seul nucléotide, la détection d'agents pathogènes et la détection de cellules et d'organismes génétiquement modifiés. Ces méthodes peut être mises en oeuvre à partir de préparations purifiées d'acides nucléiques ou de lysats cellulaires non purifiés. L'invention concerne également des méthodes de diagnostic et des nécessaires permettant de mettre en oeuvre lesdites méthodes.

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