Expression in yeast of antigenically active, recombinant...

Details Expression in yeast of antigenically active, recombinant... Expression in yeast of antigenically active, recombinant...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) A61K 38/51 (2006.01) C12N 9/88 (2006.01) C12N 15/63 (2006.01) C09J 7/02 (2006.01) C09J 123/08 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2278787

An antigenically active hybrid glutamic acid decarboxylase (GAD) comprising an amino-terminal moiety derived from the GAD67 isoform linked directly or indirectly with a middle and carboxy-terminal moiety derived from the GAD65 isoform, and production thereof as a recombinant protein by expression in eukaryotic host cells, particularly yeasts.

Cette décarboxylase hybride, active sur le plan antigénique, de l'acide glutamique (GAD) comprend une fraction terminale amino, dérivée de l'isoforme GAD67 liée directement ou indirectement à une fraction intermédiaire et terminale carboxy dérivée de l'isoforme GAD65. L'invention concerne la production de cette décarboxylase en tant que protéine de recombinaison, par expression de celle-ci dans des cellules hôtes eucaryotes, notamment des levures.

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