Fluorescent hybridization probes with reduced background

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2433473

The invention is directed to a pair of FRET hybridization probes consisting of a first oligonucleotide carrying a FRET donor entity and a second oligonucleotide carrying a FRET acceptor entity, wherein said first oligonucleotide carrying the donor fluorescent entity is carrying at least one second entity, said second entity being a compound which is capable of quenching fluorescence emission of said donor fluorescent entity. In addition, the invention is directed to methods of using the same.

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