Food additives

C - Chemistry – Metallurgy – 12 – N

Patent

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99/22, 195/1.17,

C12N 15/63 (2006.01) A23L 1/03 (2006.01) A23L 3/3571 (2006.01) A23L 3/37 (2006.01) A23L 3/44 (2006.01) C07K 14/21 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/74 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2027852

TITLE : FOOD ADDITIVES INVENTORS : H. HOTTINGER P. NIEDERBERGER D. PRIDMORE U. STAEGER-ROOSE ABSTRACT : Additives for promoting ice nucleation in ingestible biological materials at sub-zero temperatures comprising a protein capable of promoting said ice nucleation carried by GRAS microorganisms transformed by a vector carrying one or more genes coding for the expression of said protein or by fractions of said microorganisms. Said vector is a plasmid comprising in the correct reading frame a cloning vector and DNA coding for said protein capable of promoting ice-nucleation and in the ease of a yeast GRAS microorganism substantially the complete yeast 2 micron plasmid, at least one yeast selectable auxotrophic marker and the dLEU2 allele of the LEU2 gene and, in the case of a Lacto- coccal GRAS microorganism, additionally a non-inducible erythromycin resistance gene and a lactococcal plasmid promoter and origin of replication.

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