C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 13/00 (2006.01) A61K 9/127 (2006.01) A61K 9/133 (2006.01) B01J 13/00 (2006.01) C07F 9/10 (2006.01) C07H 15/04 (2006.01) C07H 15/08 (2006.01) C12P 7/20 (2006.01) C12P 19/00 (2006.01)
Patent
CA 2122638
Novel ether lipids were obtained from methanogenic (Methanospirillum hungatei, Methanococcus jannaschii, Methanococcus voltae. Methanosarcina mazei, and Methanobrevibacter smithii), and extremely halophilic (Halobacterium cutirubrum) representatives of the archaeobacteria. Several of the ether lipids produced by Methanospirillum and Methanosarcina genera were purified and characterized structurally for the first time. Unilamellar liposomes were prepared from emulsions of the total polar-ether lipid extracts of such bacteria by pressure extrusion through membranes of various pore sizes. Liposome populations were shown by dynamic light scattering and electron microscopy to range in size depending on the pore size of the filter, on the source of the lipids, and on the composition of the suspending buffer medium. In all cases the size ranges indicated highly homogeneous preparations. Leakage of entrapped fluorescent or radioactive compounds established that the ether liposomes were stable to attack by phospholipase A2 and B, and were stable for at least 60 days to storage in an atmosphere of air. The detergent dialysis method of forming liposomes also produced unilamellar liposomes from most of the archaeobacterial total polar lipid extracts which were tested.
Des représentants méthanogènes (Methanospirillum hungatei, Metanococcus jannaschii, Methanococcus voltae, Methanosarcina mazei et Methanobrevibacter smithii), et d'autres, très halophiles (Halobacterium cutirubrum), d'archéobactéries donnent de nouveaux lipides d'éthers. Plusieurs de ces derniers, produits par les genres Methanospirillum et Methanosarcina ont été purifiés et structurellement caractérisés pour la première fois. On a préparé des liposomes unilamellaires à partir d'émulsions d'extraits contenant des lipides polaires totaux et de lipides d'éthers provenant de ces bactéries par extrusion sous pression au travers de membranes dotées de pores de différentes dimensions. La diffusion lumineuse dynamique et la microscopie électronique ont montré que les populations de liposomes étaient de taille variable selon la dimension des pores du filtre utilisé pour la source de lipides, et selon la composition du milieu tampon de suspension. Dans tous les cas, les fourchettes concernant ces dimensions témoignaient de préparations très homogènes. Des fuites de composés piégés, de nature fluorescente ou radioactive, ont prouvé que les liposomes d'éthers résistaient à l'attaque des phospholipases A2 et B et restaient stables au moins 60 jours en cas de stockage à l'air libre. Le procédé de dialyse pour détergents a aussi permis de créer des liposomes unilamellaires à partir de la plupart des extraits de lipides polaires totaux d'archéobactéries qui ont fait l'objet
Choquet Christian G.
Ekiel Irena
Patel Girishchandra B.
Sprott G. Dennis
Anderson J. Wayne
Choquet Christian G.
Ekiel Irena
National Research Council Of Canada
Patel Girishchandra B.
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