Gene for vitamin d hydroxylase

Details Gene for vitamin d hydroxylase Gene for vitamin d hydroxylase

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12P 7/02 (2006.01)

Patent

CA 2138516

From the cells of Actinomycetes belonging to the genus Nocardia or the genus Amycolata, there is obtained a gene for expressing a hydroxylase activity capable of converting the hydrogen atom at the 25-position of a vitamin D compound having a hydrogen atom at the 25-position into a hydroxyl group. By transforming Streptomyces lividans or Streptomyces griseus with a plasmid bearing the gene and treating vitamin D compounds having a hydrogen atom at the 25-position with the transformant, vitamin D compounds introduced a hydroxyl group at the 25-position can be obtained efficiently in a high yield.

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